Organovo’s bioprinting process centers around the identification of key architectural and compositional elements of a target tissue, and the creation of a design that can be utilized by a bioprinter to generate that tissue in the laboratory environment.
Once a tissue design is established, the first step is to develop the bioprocess protocols required to generate the multi-cellular building blocks—also called bio-ink—from the cells that will be used to build the target tissue.
The bio-ink building blocks are then dispensed from a bioprinter, using a layer-by-layer approach that is scaled for the target output. Bio-inert hydrogel components may be utilized as supports, as tissues are built up vertically to achieve three-dimensionality, or as fillers to create channels or void spaces within tissues to mimic features of native tissue.
The bioprinting process can be tailored to produce tissues in a variety of formats, from micro-scale tissues contained in standard multi-well tissue culture plates, to larger structures suitable for placement onto bioreactors for biomechanical conditioning prior to use.
See how we create functional human tissues starting with cells from any source.