Compound-induced hepatotoxicity leading to fibrosis remains a challenge for human risk assessment. Latency to detection and lack of early biomarkers make it difficult to characterize the dynamic and complex intercellular interactions that occur during progressive liver injury. Thus in vitro systems that recapitulate this cellular complexity in a three dimensional context offer advantages over simple monoculture systems with respect to predictive modeling. Through the incorporation of key architectural features and primary cell types, 3D bioprinted liver can be maintained in culture for greater than four weeks, making it an ideal platform for studying phenotypes that arise during recurring exposure. Here, we discuss the utility of NovoView™ Human Liver Tissue for modeling chronic compound exposure using fibrosis as a case study and provide a comprehensive approach to examine key initiating events and progression of tissue injury.