A 3D Bioprinted Model of the Renal Proximal Tubulointerstitial Interface for Evaluation of Drug-Induced Toxicity
Presented live on September 29, 2016
Presenter: Shelby M. King, Ph.D., Senior Research Scientist, Organovo
Moderator: Tom Murphy, Ph.D., Director, Scientific Applications, Organovo
Due to its exposure to high concentrations of xenobiotics, the kidney proximal tubule (PT) is a primary site of nephrotoxicity, a leading cause of attrition in the drug development pipeline. Current preclinical methods using 2D cell cultures and animal models are unable to fully recapitulate clinical drug responses due to limited in vitro functional lifespan, or species-specific differences. Our NovoGen Bioprinter® Technology offers an opportunity to build in vitro tissue models to enable more accurate prediction of clinical outcomes.
In this webinar we describe:
- ExVive™ 3D Bioprinted Kidney Tissue, a fully human primary cell model of the PT composed of renal fibroblasts, endothelial cells, and polarized human renal PT epithelial cells (RPTEC).
- Histological and functional characterization of the tissue.
- Responses to several different classes of nephrotoxic compounds, including a case study on cisplatin toxicity and rescue by the OCT2 inhibitor cimetidine.
Dr. Shelby King has spent more than 8 years studying the impacts of 3D culture conditions on epithelial cell biology. In her role as a Senior Research Scientist at Organovo, her research focuses on the use of additive manufacturing to create 3D culture systems for studying key questions in kidney physiology and toxicology as well as cancer cell biology and the tumor microenvironment. Dr. Shelby King holds a Ph.D. from Northwestern University in Cell and Molecular Biology.