Creating a custom 3D tissue model begins with a conversation where we define your research or therapeutic needs and discuss the possible formats and delivery methods. To help you start thinking about what you can do with 3D human tissues, we’ve outlined the three key design parameters below, which are related to both research and therapeutic applications.
Three Key Design Parameters for Successful Tissue Modeling
What cell inputs do you want to focus on? Some organizations have experience with a particular cell source and can communicate to us exactly what they need; in other cases we provide assistance to determine the inputs that are most appropriate for their project. We can create tissues from a wide variety of cellular inputs, including primary human cells, cell lines, iPS-derived cells, or when available, patient samples, which are critical for the study of rare diseases and genetic disorders. For therapeutic tissues, the ability to use autologous cells (a patient’s own cells) can potentially eliminate the risk of transplant rejection and the need for life-long immunosuppressant drugs.
Some of the questions we will discuss are: How many cell types are needed? What is the micro-architecture—how will we define the spatial relationship between the different types of cells? What is the desired format? For example, multiwell plates, or pieces of tissue in a particular shape, such as a block or tube. Are other attributes required?
Assay Design / Delivery Method
What questions do you want to ask of this tissue? For in vitro tissue models, we work from the outset to define the assays that are built into the system. For therapeutic tissues, what mode of tissue delivery will maximize the therapeutic effect? Possible ways to deliver therapeutic tissues include: grafting onto an organ; implanting peritoneally or subcutaneously; or delivering therapy via extracorporeal tissues (a dialysis-like approach). What construction will be most effective at enabling the new tissue to engraft with the patient’s own tissue? For more information about custom tissue partnerships, contact us.